Vice Chancellor for Research

Molecular Resource Center

Affymetrix Microarrays

Affymetrix Microarrays Guidelines
Procedure	Sample type	Buffer to Resuspend in	Concentration Needed Minimum
Expression arrays (≥1µg total RNA or ≥ 200ng mRNA)	RNA (total or mRNA)	Water	125ng/µl total RNA 25ng/µl mRNA
Expression arrays (10-100ng total RNA)	RNA (total or mRNA)	Water	3.5ng/µl
Prokaryotic End Labeling	RNA (total or mRNA)	Water	555ng/µl (10µg’s RNA needed)
Prokaroytic Amplification	RNA (total or mRNA)	Water	100ng/µl total RNA 40ng/µl mRNA
Whole Transcriptome Exon Array	RNA (total)	Water	310 ng/µl recommended for optimal expression (33.5ng/µl can be used for small sample sizes for samples with limited amounts of RNA)
Whole Transcriptome Gene ST 1.0 array	RNA (total)	Water	33.5 ng/µl (recommend 100ng/µl)
Tiling Arrays	RNA (total)	Water	400 ng/µl - Human and Mouse 1µg/µl - all other organisms
ChIP on Chip	DNA	Water	1 ng/µl in 10µl water minimum
miRNA	RNA (total)	Water	125 ng/µl recommended 12.5 ng/µl minimum
Genotyping	DNA	Reduced EDTA TE buffer	50ng/µl
Mapping	DNA	Reduced EDTA TE buffer	50ng/µl
SNP	DNA	Reduced EDTA TE buffer	50ng/µl
Resequencing Arrays	DNA	Water	5ng/µl in 20 µl water minimum

Please follow guidelines above for dilutions of samples for Affymetrix arrays.

NOTE: All RNA samples need to be DNased to remove genomic DNA contamination.

Sample quality will be checked using Agilent Bioanalyzer, RNA samples need to be at a minimum 50ng/µl and for pico chips you will need to contact MRC staff for appropriate concentration.

For sample sizes below the minimum concentrations mentioned above or if you have any questions please contact Lorne Rose at 901-448-8229.

For pricing click MRC Affymetrix Microarrays Price List.

Additional information about Affymetrix arrays please visit the Affymetrix website